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SKU(재고 관리 코드):YXX04901
PNGase F
PNGase F
Catalog No.: YXX04901
Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins
| Catalog No. | YXX04901 |
|---|---|
| Product name | PNGase F |
| Size | 175 KU |
| Expression system | E. coli |
| Protein length | PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli. |
| Predicted molecular weight | 37.08 kDa |
| Description | Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins |
| Form | Liquid |
| Storage buffer | 20mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM Na2EDTA, 50% glycerol |
| Purity | >95% as determined by SDS-PAGE. |
| Applications | Removal of high mannose N-glycans from glycoproteins |
| Biological activity | One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 μl. |
| Endotoxin level | Please contact with the lab for this information. |
| Nature | Recombinant |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| Species | Elizabethkingia miricola |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note | For research use only. |
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