AntibodySystem
SKU(재고 관리 코드):YXX26701
Lba Cas12a (Cpf1) Nuclease
Lba Cas12a (Cpf1) Nuclease
Catalog No.: YXX26701
Lba Cas12a nuclease is derived from Lachnospiraceae bacterium ND2006 and is a site-specific DNA endonuclease mediated by a single-guide RNA (gRNA) with a length of 41-44 nucleotides. In the presence of a PAM (TTTN) sequence on the target double-stranded DNA, LbCas12a specifically cleaves the target dsDNA, resulting in double-strand breaks and the generation of sticky ends. In contrast, the specific cleavage of single-stranded DNA targets by LbCas12a does not require a PAM sequence. \r\n
Lba Cas12a contains simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N-terminus and C-terminus. When Cas12a is expressed with these NLS sequences, the Cas12a-RNP complex can rapidly localize to the nucleus upon cellular entry. \r\n
Additionally, both double-stranded and single-stranded DNA targets can activate the trans-cleavage activity of LbCas12a. Specifically, upon formation of a ternary complex among the LbCas12a enzyme, crRNA, and target DNA, the enzyme becomes activated and exhibits non-specific trans-cleavage activity, degrading any single-stranded DNA (ssDNA) present in the system. Consequently, LbCas12a can not only be used for specific cleavage of dsDNA in vitro but also enables rapid detection of target nucleic acids.
| Catalog No. | YXX26701 |
|---|---|
| Product name | Lba Cas12a (Cpf1) Nuclease |
| Size | 200 μl |
| Description | Lba Cas12a nuclease is derived from Lachnospiraceae bacterium ND2006 and is a site-specific DNA endonuclease mediated by a single-guide RNA (gRNA) with a length of 41-44 nucleotides. In the presence of a PAM (TTTN) sequence on the target double-stranded DNA, LbCas12a specifically cleaves the target dsDNA, resulting in double-strand breaks and the generation of sticky ends. In contrast, the specific cleavage of single-stranded DNA targets by LbCas12a does not require a PAM sequence. \r\n Lba Cas12a contains simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N-terminus and C-terminus. When Cas12a is expressed with these NLS sequences, the Cas12a-RNP complex can rapidly localize to the nucleus upon cellular entry. \r\n Additionally, both double-stranded and single-stranded DNA targets can activate the trans-cleavage activity of LbCas12a. Specifically, upon formation of a ternary complex among the LbCas12a enzyme, crRNA, and target DNA, the enzyme becomes activated and exhibits non-specific trans-cleavage activity, degrading any single-stranded DNA (ssDNA) present in the system. Consequently, LbCas12a can not only be used for specific cleavage of dsDNA in vitro but also enables rapid detection of target nucleic acids. |
| Form | Liquid |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | Genome Editing, DNA Detection, CRISPR Diagnostics |
| Endotoxin level | Please contact with the lab for this information. |
| Nature | Recombinant |
| Stability and Storage | Store at -20°C for twelve months from the date of receipt. |
| Alternative Names | CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1, Lba Cas12a (Cpf1), LbaCas12a, Cpf1, LbCas12a |
| Species | Lachnospiraceae bacterium ND2006 |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note | For research use only. |
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