ACTR3; ARP3; Actin-related protein 3; Actin-like protein 3
Applications:
WB;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human ACTR3. AA range:369-418
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
47kD
GeneID（Human）:
10096
Human Swiss-Prot No:
P61158
Cellular localization:
Cytoplasm, cytoskeleton . Cell projection . Nucleus . In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) (PubMed:19109554).
Background:
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013],
Western Blot analysis of various cells using Arp3 Polyclonal Antibody
Western Blot analysis of K562 cells using Arp3 Polyclonal Antibody
Western blot analysis of lysates from Jurkat and RAW264.7 cells, using ACTR3 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from COLO205 cells using ACTR3 antibody.