ASK 1 Polyclonal Antibody

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

The AS250 Polyclonal Antibody is a highly specific and sensitive immunological tool designed for the detection and quantification of target proteins in various biological samples. This antibody is produced by immunizing animals with a purified antigen, resulting in a polyclonal antibody that recognizes multiple epitopes on the target protein.

The AS250 Polyclonal Antibody exhibits exceptional affinity and selectivity, ensuring accurate and reliable results in a wide range of applications, including Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA). Its robust performance allows for the detection of even low-abundance target proteins, making it an invaluable tool for researchers in the fields of molecular biology, biochemistry, and biomedicine.

With its superior sensitivity, the AS250 Polyclonal Antibody enables the detection of target proteins at low concentrations, minimizing the need for sample manipulation and reducing the risk of false negatives. Its high specificity ensures minimal cross-reactivity with non-target proteins, enhancing the accuracy and reliability of experimental data.

The AS250 Polyclonal Antibody is supplied as a ready-to-use solution, eliminating the need for time-consuming antibody purification or conjugation steps. Its long shelf life and stability make it a cost-effective choice for laboratories of all sizes.

In summary, the AS250 Polyclonal Antibody is a highly reliable and versatile immunological tool that offers exceptional sensitivity, specificity, and ease of use. Its superior performance and wide range of applications make it an essential component of any research project involving the detection and quantification of target proteins.