ATF-1 Polyclonal Antibody

ATF1 encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of ATF1 and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. ATF1 has a pseudogene on chromosome 6.

The ATF-1 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Activating Transcription Factor 1 (ATF-1) in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human ATF-1 protein. The resulting polyclonal antibody is purified using protein A affinity chromatography and validated for its specificity and sensitivity through various assays.

The ATF-1 Polyclonal Antibody is suitable for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been shown to detect endogenous levels of ATF-1 protein in human, mouse, and rat tissues and cell lines. This antibody is also capable of detecting phosphorylated ATF-1 protein, making it a valuable tool for studying the regulation of ATF-1 activity.

The ATF-1 Polyclonal Antibody is provided as a liquid solution in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. It is available in various sizes to meet the needs of different research projects. This antibody is manufactured under strict quality control standards to ensure consistent performance and reproducibility.

In summary, the ATF-1 Polyclonal Antibody is a reliable and high-quality tool for the detection of ATF-1 protein in various biological samples. Its specificity, sensitivity, and versatility make it an ideal choice for researchers studying the role of ATF-1 in various cellular processes.