ATF-5 Polyclonal Antibody

Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB3 and Luman), CREM-1, CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-5 (ATFx), which can localize to the cytoplasm or the nucleus, binds DNA as a dimer. It interacts with CCND3 and PTP4A1.

The ATF-5 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of ATF-5 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human ATF-5 protein. The resulting polyclonal antibody exhibits high affinity and specificity towards the target protein, making it an ideal tool for various applications such as Western blotting, immunohistochemistry, and immunofluorescence.

The ATF-5 Polyclonal Antibody is validated for use in human, mouse, and rat samples, and has been extensively tested to ensure consistent and reliable results. This antibody is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. It is recommended to store the antibody at -20°C for long-term storage and to avoid repeated freeze-thaw cycles.

Overall, the ATF-5 Polyclonal Antibody is a valuable tool for researchers studying the role of ATF-5 protein in various biological processes, including cell proliferation, differentiation, and apoptosis. Its high specificity and sensitivity make it an excellent choice for detecting ATF-5 protein in complex biological samples.