Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human ATP-Citrate Lyase. AA range:420-469
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
120kD
GeneID（Human）:
47
Human Swiss-Prot No:
P53396
Cellular localization:
Cytoplasm, cytosol .
Background:
ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014],
Western Blot analysis of various cells using ATP-citrate synthase Polyclonal Antibody diluted at 1:1000
Western Blot analysis of COS7 cells using ATP-citrate synthase Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from COS7 cells, treated with Calyculin 50nM 30', using ATP-Citrate Lyase Antibody. The lane on the right is blocked with the synthesized peptide.