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Biospes
SKU(재고 관리 코드):BEK1233
Human VEGFR2 ELISA Kit
Human VEGFR2 ELISA Kit
Application: For quantitative detection of VEGFR2 in human serum,plasma, urine, cell culture supernatant or tissue samples.
Introduction: VEGF receptors are receptors for vascular endothelial growth factor (VEGF). They play an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis.There are three main subtypes of VEGFR, numbered 1, 2 and 3. VEGFR2, also known as Kinase insert domain receptor (KDR) or Flk1 (Fetal Liver Kinase 1), plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Furthermore, therapeutic inhibition of VEGFR-2 action is now having an impact in the clinic for the treatment of a number of diseases.
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-VEGFR2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-VEGFR2antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VEGFR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VEGFR2 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months.
Range: 30 pg/ml - 800 pg/ml
Sensitivity:
Introduction: VEGF receptors are receptors for vascular endothelial growth factor (VEGF). They play an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis.There are three main subtypes of VEGFR, numbered 1, 2 and 3. VEGFR2, also known as Kinase insert domain receptor (KDR) or Flk1 (Fetal Liver Kinase 1), plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Furthermore, therapeutic inhibition of VEGFR-2 action is now having an impact in the clinic for the treatment of a number of diseases.
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-VEGFR2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-VEGFR2antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VEGFR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VEGFR2 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months.
Range: 30 pg/ml - 800 pg/ml
Sensitivity:
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