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Biospes

SKU(재고 관리 코드):BEK1244

Mouse Insulin ELISA Kit

Mouse Insulin ELISA Kit

Size
Application: For quantitative detection of in sul in in mouse serum,plasma, body fluids, tissuelysates or cell culture supernates.

Introduction: Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The human insulin gene contains 3 exons; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Insulin has a potent acute antiinflammatory effect, including a reduction in intranuclear NF-kappa-B, an increase in IKB, and decreases in the generation of reactive oxygen species. It causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen inside these tissues,and improved insulin-stimulated glucose uptake after endurance training results from hemodynamic adaptations as well as increased cellular protein content of individual insulin signaling components and molecules involved in glucose transport and metabolism.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Insulin monoclonalantibodywaspre-coated onto 96-well plates. And theHRP conjugated anti-Insulin polyclonal antibody was used as detection antibodies. The standards, test samples andHRPconjugated detection antibody were added to the wells subsequently, and wash with wash buffer.TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Insulin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Insulin can be calculated.

Storage and Expiration: Store at 4℃ for 12 months.

Range: 0 uIU/ml - 140 uIU/ml

Sensitivity: < 5 uIU/ml
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