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Biospes
SKU(재고 관리 코드):BEK1254
Human Fatty acid-binding protein, heart, HFABP / FABP3 ELISA Kit
Human Fatty acid-binding protein, heart, HFABP / FABP3 ELISA Kit
Application: For quantitative detection of human HFABP in serum,cell culture supernatants or tissue homogenate.
Introduction: Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in human s is encoded by the FABP3 gene. The serum HFABP level is an effective biomarker for detecting AKI in non-surgical intensive care patients. HFABP expression is significantly higher in in-stent restenosis, ISR tissue than in non-ISR tissue from diabetic and nondiabetic minipigs. Overexpression of HFABP induces multiple pathway-related promotion of inflammation, growth and migration in vascular SMCs, suggesting a potential role in coronary artery ISR. hFABP is a rapid marker of perioperative myocardial damage and peaks earlier than CKMB or TnI. The kinetics of marker proteins in serial samples immediately after reperfusion is more suitable for the detection of perioperative myocardial infarction than a fixed cut-off level.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HFABPmonoclonalantibodywaspre-coated onto 96-well plates. The standards andtest samples were added to the wellsand anyHFABPpresent is bound by the immobilized antibody. After washing away any unbound substances,theHRP conjugated anti-HFABPmonoclonal antibody wasaddedto wells as detection antibodies. Following a wash to remove any unbound antibody-enzyme reagent, the TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding stop solution. The density of yellow is proportional to the HFABP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HFABP can be calculated.
Storage and Expiration: Storeat 4℃ for 6 months, or at -20℃ for one year.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: 1 pg/ml
Introduction: Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in human s is encoded by the FABP3 gene. The serum HFABP level is an effective biomarker for detecting AKI in non-surgical intensive care patients. HFABP expression is significantly higher in in-stent restenosis, ISR tissue than in non-ISR tissue from diabetic and nondiabetic minipigs. Overexpression of HFABP induces multiple pathway-related promotion of inflammation, growth and migration in vascular SMCs, suggesting a potential role in coronary artery ISR. hFABP is a rapid marker of perioperative myocardial damage and peaks earlier than CKMB or TnI. The kinetics of marker proteins in serial samples immediately after reperfusion is more suitable for the detection of perioperative myocardial infarction than a fixed cut-off level.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HFABPmonoclonalantibodywaspre-coated onto 96-well plates. The standards andtest samples were added to the wellsand anyHFABPpresent is bound by the immobilized antibody. After washing away any unbound substances,theHRP conjugated anti-HFABPmonoclonal antibody wasaddedto wells as detection antibodies. Following a wash to remove any unbound antibody-enzyme reagent, the TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding stop solution. The density of yellow is proportional to the HFABP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HFABP can be calculated.
Storage and Expiration: Storeat 4℃ for 6 months, or at -20℃ for one year.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: 1 pg/ml
