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Biospes
SKU(재고 관리 코드):BEK1255
Human Progastrin-releasing peptide, ProGRP ELISA Kit
Human Progastrin-releasing peptide, ProGRP ELISA Kit
Application: For quantitative detection of human ProGRP in serum, plasma,cell culture supernatants or tissue homogenate.
Introduction: Progastrin-releasing peptide (proGRP) is a recently identified biomarker of small cell lung cancer (SCLC). Plasma proGRP level could be a useful diagnostic and therapeutic monitoring biomarker for patients with SCLC and the initial level may help with SCLC tumor staging. ProGRP was highly expressed in NCI-H446 cells and xenograft tissue. It could be used as a marker for quality control in sample processing and storage in biobanks. Korse CM1 et al study concluded that a high-risk proGRP level (more than twice the upper reference value) in patients with well-differentiated neuroendocrine tumours, WDNETs is a strong indication for a primary tumour in the lung. Besides CgA, proGRP is a complementary tumour marker for prognosis and treatment monitoring in patients with neuroendocrine tumour.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ProGRPmonoclonalantibodywaspre-coated onto 96-well plates. The standards andtest samples were added to the wellsand anyProGRPpresent is bound by the immobilized antibody. After washing away any unbound substances,theHRP conjugated anti-ProGRPmonoclonal antibody wasaddedto wells as detection antibodies. Following a wash to remove any unbound antibody-enzyme reagent, the TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding stop solution. The density of yellow is proportional to the ProGRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ProGRP can be calculated.
Storage and Expiration: Storeat 4℃ for 6 months, or at -20℃ for one year.
Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: 2 pg/ml
Introduction: Progastrin-releasing peptide (proGRP) is a recently identified biomarker of small cell lung cancer (SCLC). Plasma proGRP level could be a useful diagnostic and therapeutic monitoring biomarker for patients with SCLC and the initial level may help with SCLC tumor staging. ProGRP was highly expressed in NCI-H446 cells and xenograft tissue. It could be used as a marker for quality control in sample processing and storage in biobanks. Korse CM1 et al study concluded that a high-risk proGRP level (more than twice the upper reference value) in patients with well-differentiated neuroendocrine tumours, WDNETs is a strong indication for a primary tumour in the lung. Besides CgA, proGRP is a complementary tumour marker for prognosis and treatment monitoring in patients with neuroendocrine tumour.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ProGRPmonoclonalantibodywaspre-coated onto 96-well plates. The standards andtest samples were added to the wellsand anyProGRPpresent is bound by the immobilized antibody. After washing away any unbound substances,theHRP conjugated anti-ProGRPmonoclonal antibody wasaddedto wells as detection antibodies. Following a wash to remove any unbound antibody-enzyme reagent, the TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding stop solution. The density of yellow is proportional to the ProGRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ProGRP can be calculated.
Storage and Expiration: Storeat 4℃ for 6 months, or at -20℃ for one year.
Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: 2 pg/ml
