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Biospes
SKU(재고 관리 코드):BEK1256
Chicken HPV16L1 Ab ELISA Kit
Chicken HPV16L1 Ab ELISA Kit
Application: For qualitativedetection of HPV16L1 Ab in Chickenserum, egg white or chicken derived samples.
Introduction: Papillomaviridae is an ancient taxonomic family of non-enveloped DNA viruses, collectively known as papillomaviruses. Antibodies to HPV16 proteins are associated with increased risk for HPVOPC. Among patients with OPC, HPV16 Abs are associated with tumor HPV status, in particular among HPV positive patients with no or little smoking history. The amino (N) terminus of the human papillomavirus (HPV) minor capsid protein L2 can induce low-titer, cross-neutralizing antibodies. Immunization withHPV16L1-HPV16 L2 (chimera 17-36) VLP in adjuvant applicable for human use induces broad-spectrum neutralizing antibodies against HPV types evolutionarily divergent to HPV16 and thus may protect against infection with mucosal high-risk, low-risk, and beta HPV types and associated disease.
Principle of the Assay: This kit was based onindirect enzyme-linked immune-sorbent assay technology. TherecombinantHPV16L1antigenswerepre-coated onto 96-well plates.The test samples were added to the wells and incubated subsequently, theHPV16L1antibodies in the test samples combined to the pre-coated antigens specifically, then followed by washing with wash buffer to remove unspecific components.And the HRP conjugated antibodies wereadded and incubated to bind tochickenantibodies in plate, the unbound conjugates were washed away with wash buffer.TMB substratewere used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader.
Storage and Expiration: Store at 2-8℃ for 6 months, at -20℃ for 12 months.Store HRP conjugat ed antibody at -20℃ for one year.
Range: Qualitative Kit
Sensitivity:
Introduction: Papillomaviridae is an ancient taxonomic family of non-enveloped DNA viruses, collectively known as papillomaviruses. Antibodies to HPV16 proteins are associated with increased risk for HPVOPC. Among patients with OPC, HPV16 Abs are associated with tumor HPV status, in particular among HPV positive patients with no or little smoking history. The amino (N) terminus of the human papillomavirus (HPV) minor capsid protein L2 can induce low-titer, cross-neutralizing antibodies. Immunization withHPV16L1-HPV16 L2 (chimera 17-36) VLP in adjuvant applicable for human use induces broad-spectrum neutralizing antibodies against HPV types evolutionarily divergent to HPV16 and thus may protect against infection with mucosal high-risk, low-risk, and beta HPV types and associated disease.
Principle of the Assay: This kit was based onindirect enzyme-linked immune-sorbent assay technology. TherecombinantHPV16L1antigenswerepre-coated onto 96-well plates.The test samples were added to the wells and incubated subsequently, theHPV16L1antibodies in the test samples combined to the pre-coated antigens specifically, then followed by washing with wash buffer to remove unspecific components.And the HRP conjugated antibodies wereadded and incubated to bind tochickenantibodies in plate, the unbound conjugates were washed away with wash buffer.TMB substratewere used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader.
Storage and Expiration: Store at 2-8℃ for 6 months, at -20℃ for 12 months.Store HRP conjugat ed antibody at -20℃ for one year.
Range: Qualitative Kit
Sensitivity: