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Biospes
SKU(재고 관리 코드):BEK1261
Human Haptoglobin / Zonulin ELISA Kit
Human Haptoglobin / Zonulin ELISA Kit
Application: For quantitative detection of Haptoglob in in human serum, plasma, body fluids, tissue homogenate or cell culture supernatants.
Introduction: Haptoglobin (Hp) is an abundant and conserved plasma glycoprotein, which binds acellular adult hemoglobin (Hb) dimers with high affinity and facilitates their rapid clearance from circulation after hemolysis. human s possess three main phenotypes of Hp, designated Hp 1-1, Hp 2-1, and Hp 2-2. These variants exhibit diverse structural configurations and have been reported to be functionally nonequivalent. Hp fractions examined are equivalent to one another with respect to Hb binding and associated stability and redox properties and that this result should be taken into account in the design of phenotype-specific Hp therapeutics aimed at countering Hb-mediated vascular disease. Hp scavenges free hemoglobin following malaria-induced hemolysis.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Haptoglobin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Haptoglobin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Haptoglobin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Haptoglobin can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 312 pg/ml - 20,000 pg/ml
Sensitivity: < 20 pg/ml
Introduction: Haptoglobin (Hp) is an abundant and conserved plasma glycoprotein, which binds acellular adult hemoglobin (Hb) dimers with high affinity and facilitates their rapid clearance from circulation after hemolysis. human s possess three main phenotypes of Hp, designated Hp 1-1, Hp 2-1, and Hp 2-2. These variants exhibit diverse structural configurations and have been reported to be functionally nonequivalent. Hp fractions examined are equivalent to one another with respect to Hb binding and associated stability and redox properties and that this result should be taken into account in the design of phenotype-specific Hp therapeutics aimed at countering Hb-mediated vascular disease. Hp scavenges free hemoglobin following malaria-induced hemolysis.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Haptoglobin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Haptoglobin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Haptoglobin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Haptoglobin can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 312 pg/ml - 20,000 pg/ml
Sensitivity: < 20 pg/ml
