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Biospes
SKU(재고 관리 코드):BEK1265
Anti-SARS-CoV-2 Neutralizing Antibody(NtAb) ELISA Kit
Anti-SARS-CoV-2 Neutralizing Antibody(NtAb) ELISA Kit
Application: For qualitative detection of any antibodies that neutralize the in teraction of SARS-CoV-2 Spike RBD-ACE2 in serum, plasma.
Introduction: Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2,or 2019-nCoV) is anenveloped non-segmented positive-sense RNA virus. It is the cause of coronavirus disease 2019(COVID-19),which is contagious in human s.SARS-CoV-2 has several structural proteins including spike (S), envelope (E),membrane (M)and nucleocapsid (N). The spike protein (S) contains a receptor binding domain (RBD), which isresponsible for recognizing the cell surface receptor, angiotensin converting enzyme-2 (ACE2). Itis found that the RBD of the SARS-CoV-2 S protein strongly interacts with the human ACE2receptor leading to endocytosis into the host cells of the deep lung and viral replication.Infection with the SARS-CoV-2 initiates an immune response, which includes the productionof antibodies in the blood. The secreted antibodies provide protection against future infectionsfrom viruses, because they remain in the circulatory system for months to years after infection andwill bind quickly and strongly to the pathogen to block cellular infiltration and replication. Theseantibodies are named neutralizing antibodies. This kit can detect circulating neutralizing antibodies against SARS-CoV-2 that block the interaction between the receptor binding domain of the viral spike glycoprotein (RBD) with the ACE2 cell surface receptor. The detection is not limited by both species and antibody isotypes.
Principle of the Assay: The kit isbased on the competitive ELISA, it isa blocking ELISA detection tool, which mimics the virus neutralization process. The kit contains two key components:HRPconjugated recombinant SARS-CoV-2SpikeRBD fragmentprotein(HRP-SRBD)and the human ACE2 receptor protein (hACE2). The protein-proteininteractionsbetween HRP-SRBD and hACE2 can be blocked by neutralizing antibodies against SARS-CoV-2SpikeRBD.Incubatethe samplesandcontrolswith HRP-SRBDtomakethecombinationof the neutralization antibodieswithHRP-SRBD. Then, add themixtureto thehACE2 pre-coatedplate. The unboundHRP-SRBDas well as anyHRP-SRBDbound to non-neutralizing antibody will be captured on the plate, while the neutralizingantibodies-HRP-SRBDcomplexes remain in the supernatant andwere washed away withwash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is inverse to theAnti-SARS-CoV-2 Neutralizing Antibody amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Anti-SARS-CoV-2 Neutralizing Antibody can be calculated. Figure 1: From Chee Wah Tan, Wan Ni Chia, Mark I-C Chen, Zhiliang Hu, Barnaby E. Young, Yee-Joo Tan, Yongxiang Yi, David C. Lye, Danielle E. Anderson, Lin-Fa Wang. A SARS-CoV-2 surrogate virus neutralization test (sVNT) based on antibody-mediated blockage of ACE2-spike (RBD) protein-protein interaction. DOI: https://doi.org/10.21203/rs.3.rs-24574/v1
Storage and Expiration: Store at 2-8℃ for 6 months.
Range: Qualitative Kit
Sensitivity:
Introduction: Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2,or 2019-nCoV) is anenveloped non-segmented positive-sense RNA virus. It is the cause of coronavirus disease 2019(COVID-19),which is contagious in human s.SARS-CoV-2 has several structural proteins including spike (S), envelope (E),membrane (M)and nucleocapsid (N). The spike protein (S) contains a receptor binding domain (RBD), which isresponsible for recognizing the cell surface receptor, angiotensin converting enzyme-2 (ACE2). Itis found that the RBD of the SARS-CoV-2 S protein strongly interacts with the human ACE2receptor leading to endocytosis into the host cells of the deep lung and viral replication.Infection with the SARS-CoV-2 initiates an immune response, which includes the productionof antibodies in the blood. The secreted antibodies provide protection against future infectionsfrom viruses, because they remain in the circulatory system for months to years after infection andwill bind quickly and strongly to the pathogen to block cellular infiltration and replication. Theseantibodies are named neutralizing antibodies. This kit can detect circulating neutralizing antibodies against SARS-CoV-2 that block the interaction between the receptor binding domain of the viral spike glycoprotein (RBD) with the ACE2 cell surface receptor. The detection is not limited by both species and antibody isotypes.
Principle of the Assay: The kit isbased on the competitive ELISA, it isa blocking ELISA detection tool, which mimics the virus neutralization process. The kit contains two key components:HRPconjugated recombinant SARS-CoV-2SpikeRBD fragmentprotein(HRP-SRBD)and the human ACE2 receptor protein (hACE2). The protein-proteininteractionsbetween HRP-SRBD and hACE2 can be blocked by neutralizing antibodies against SARS-CoV-2SpikeRBD.Incubatethe samplesandcontrolswith HRP-SRBDtomakethecombinationof the neutralization antibodieswithHRP-SRBD. Then, add themixtureto thehACE2 pre-coatedplate. The unboundHRP-SRBDas well as anyHRP-SRBDbound to non-neutralizing antibody will be captured on the plate, while the neutralizingantibodies-HRP-SRBDcomplexes remain in the supernatant andwere washed away withwash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is inverse to theAnti-SARS-CoV-2 Neutralizing Antibody amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Anti-SARS-CoV-2 Neutralizing Antibody can be calculated. Figure 1: From Chee Wah Tan, Wan Ni Chia, Mark I-C Chen, Zhiliang Hu, Barnaby E. Young, Yee-Joo Tan, Yongxiang Yi, David C. Lye, Danielle E. Anderson, Lin-Fa Wang. A SARS-CoV-2 surrogate virus neutralization test (sVNT) based on antibody-mediated blockage of ACE2-spike (RBD) protein-protein interaction. DOI: https://doi.org/10.21203/rs.3.rs-24574/v1
Storage and Expiration: Store at 2-8℃ for 6 months.
Range: Qualitative Kit
Sensitivity:
