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Biospes
SKU(재고 관리 코드):BEK1289
Human L-FABP ELISA Kit
Human L-FABP ELISA Kit
Application: For quantitative detection of L-FABP in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: Fatty acid-binding protein 1 (FABP1) also known as liver-type fatty acid-binding protein (L-FABP) is a protein that in human s is encoded by the FABP1 gene. Liver fatty acid-binding protein (L-FABP) and liver bile acid-binding protein (L-BABP), in the liver intra-cytoplasm of chicken, are members of the fatty acid-binding protein subfamily. Zhang Q et al suggested that chicken L-FABP and L-BABP affect abdominal fat deposition through differences in their expression level, and the possible mechanism is that a high expression level of L-FABP and L-BABP leads to a high lipogenesis rate and, ultimately, to lipid deposition. L-Fabp, a dominant intestinal fatty acid-binding protein, regulates intestinal fatty acid trafficking and metabolism, and L-Fabp deletion attenuates diet-induced obesity.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-L-FABP polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-L-FABP polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the L-FABP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of L-FABP can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: < 12 pg/ml
Introduction: Fatty acid-binding protein 1 (FABP1) also known as liver-type fatty acid-binding protein (L-FABP) is a protein that in human s is encoded by the FABP1 gene. Liver fatty acid-binding protein (L-FABP) and liver bile acid-binding protein (L-BABP), in the liver intra-cytoplasm of chicken, are members of the fatty acid-binding protein subfamily. Zhang Q et al suggested that chicken L-FABP and L-BABP affect abdominal fat deposition through differences in their expression level, and the possible mechanism is that a high expression level of L-FABP and L-BABP leads to a high lipogenesis rate and, ultimately, to lipid deposition. L-Fabp, a dominant intestinal fatty acid-binding protein, regulates intestinal fatty acid trafficking and metabolism, and L-Fabp deletion attenuates diet-induced obesity.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-L-FABP polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-L-FABP polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the L-FABP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of L-FABP can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: < 12 pg/ml
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