1
/
의
1
Biospes
SKU(재고 관리 코드):BEK1291
Human AMICA / JAML ELISA Kit
Human AMICA / JAML ELISA Kit
Application: For quantitative detection of AMICA / JAML in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: JAML or Junctional Adhesion Molecule-Like, or AMICA1 is a JAM transmembrane protein family member.It is composed of two extracellular immunoglobulin-like domains, a membrane-spanning region, and a cytoplasmic tail involved in activation signaling. A known ligand of JAML is Coxsackie virus and Adenovirus Receptor (CXADR in human s and CAR in mice) which has been shown to localize to the tight junctions of epithelial cells.JAML-mediated activation of CAR is required for neutrophil extravasationin addition to other leukocyte/epithelial cell interaction models.Other members of the JAM family of transmembrane proteins include JAM1, JAM2 and JAM3.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-AMICA / JAML polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AMICA / JAML polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AMICA / JAML amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AMICA / JAML can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: < 10 pg/ml
Introduction: JAML or Junctional Adhesion Molecule-Like, or AMICA1 is a JAM transmembrane protein family member.It is composed of two extracellular immunoglobulin-like domains, a membrane-spanning region, and a cytoplasmic tail involved in activation signaling. A known ligand of JAML is Coxsackie virus and Adenovirus Receptor (CXADR in human s and CAR in mice) which has been shown to localize to the tight junctions of epithelial cells.JAML-mediated activation of CAR is required for neutrophil extravasationin addition to other leukocyte/epithelial cell interaction models.Other members of the JAM family of transmembrane proteins include JAM1, JAM2 and JAM3.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-AMICA / JAML polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AMICA / JAML polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AMICA / JAML amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AMICA / JAML can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: < 10 pg/ml
Share
