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Biospes
SKU(재고 관리 코드):BEK1309
Mouse IL-11 ELISA Kit
Mouse IL-11 ELISA Kit
Application: For quantitative detection of IL-11 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: Interleukin 11 (IL-11 or adipogenesis inhibitory factor) is a protein that in human s is encoded by the IL11 gene.IL-11 is a cytokine that was first isolated in 1990 from bone marrow-derived fibrocyte-like stromal cells. It was initially thought to be important for hematopoiesis, notably for megakaryocyte maturation,but subsequently shown to be redundant for platelets, and for other blood cell types, in both mice and human s.It was developed as a recombinant protein (rhIL-11) as the drug substance oprelvekin.The mouse IL-11 gene, consisting of 5 exons and 4 introns, is located on chromosome 19, and encodes a 23 kDa protein. IL-11 is a member of the IL-6-type cytokine family, distinguished based on their use of the common co-receptor gp130.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-11 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-11 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-11 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-11 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 125 pg/ml - 8000 pg/ml
Sensitivity: < 30 pg/ml
Introduction: Interleukin 11 (IL-11 or adipogenesis inhibitory factor) is a protein that in human s is encoded by the IL11 gene.IL-11 is a cytokine that was first isolated in 1990 from bone marrow-derived fibrocyte-like stromal cells. It was initially thought to be important for hematopoiesis, notably for megakaryocyte maturation,but subsequently shown to be redundant for platelets, and for other blood cell types, in both mice and human s.It was developed as a recombinant protein (rhIL-11) as the drug substance oprelvekin.The mouse IL-11 gene, consisting of 5 exons and 4 introns, is located on chromosome 19, and encodes a 23 kDa protein. IL-11 is a member of the IL-6-type cytokine family, distinguished based on their use of the common co-receptor gp130.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-11 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-11 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-11 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-11 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 125 pg/ml - 8000 pg/ml
Sensitivity: < 30 pg/ml
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