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Biospes
SKU(재고 관리 코드):BEK1316
Human CD244 / SLAMF4 ELISA Kit
Human CD244 / SLAMF4 ELISA Kit
Application: For quantitative detection of CD244 / SLAMF4 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: CD244 (Cluster of Differentiation 244) also known as 2B4 or SLAMF4 is a protein that in human s is encoded by the CD244 gene.CD244 is a type-I transmembrane protein belonging to the signaling lymphocytic activation molecule family of receptors (SLAMF) which are expressed in different types of hematopoietic cells.CD244 plays a role in the regulation of the immune system.A ligand of CD244 is CD48 (SLAMF2). CD48 also belongs to the SLAMF, it does not have an intracellular domain and it is anchored to the plasma membrane by a GPI-anchor.Only these two receptors from the SLAMF mediate heterophilic interactions.The receptor CD244 is encoded by the CD244 gene located on the long arm of human chromosome 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CD244 / SLAMF4 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CD244 / SLAMF4 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD244 / SLAMF4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD244 / SLAMF4 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 2 pg/ml
Introduction: CD244 (Cluster of Differentiation 244) also known as 2B4 or SLAMF4 is a protein that in human s is encoded by the CD244 gene.CD244 is a type-I transmembrane protein belonging to the signaling lymphocytic activation molecule family of receptors (SLAMF) which are expressed in different types of hematopoietic cells.CD244 plays a role in the regulation of the immune system.A ligand of CD244 is CD48 (SLAMF2). CD48 also belongs to the SLAMF, it does not have an intracellular domain and it is anchored to the plasma membrane by a GPI-anchor.Only these two receptors from the SLAMF mediate heterophilic interactions.The receptor CD244 is encoded by the CD244 gene located on the long arm of human chromosome 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CD244 / SLAMF4 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CD244 / SLAMF4 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD244 / SLAMF4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD244 / SLAMF4 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 2 pg/ml
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