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Biospes
SKU(재고 관리 코드):BEK1318
Human Cathepsin S ELISA Kit
Human Cathepsin S ELISA Kit
Application: For quantitative detection of Catheps in S in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: Cathepsin S is a protein that in human s is encoded by the CTSS gene.Transcript variants utilizing alternative polyadenylation signals exist for this gene.Cathepsin S is a member of the peptidase C1 family of cysteine cathepsins, a lysosomal cysteine protease that may participate in the degradation of antigenic proteins to peptides for presentation to the MHC class II. Cathepsin S can function as an elastase over a broad pH range in alveolar macrophages.Cathepsin S is a lysosomal enzyme that belongs to the papain-like protease of cysteine proteases. While a role in antigen presentation has long been recognized, it is now understood that cathepsin S has a role in itch and pain, or nociception .
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Cathepsin S polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Cathepsin S polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Cathepsin S amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Cathepsin S can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 2 pg/ml
Introduction: Cathepsin S is a protein that in human s is encoded by the CTSS gene.Transcript variants utilizing alternative polyadenylation signals exist for this gene.Cathepsin S is a member of the peptidase C1 family of cysteine cathepsins, a lysosomal cysteine protease that may participate in the degradation of antigenic proteins to peptides for presentation to the MHC class II. Cathepsin S can function as an elastase over a broad pH range in alveolar macrophages.Cathepsin S is a lysosomal enzyme that belongs to the papain-like protease of cysteine proteases. While a role in antigen presentation has long been recognized, it is now understood that cathepsin S has a role in itch and pain, or nociception .
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Cathepsin S polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Cathepsin S polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Cathepsin S amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Cathepsin S can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 2 pg/ml
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