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SKU(재고 관리 코드):BEK1342
Mouse BAFFR/TNFRSF13C ELISA Kit
Mouse BAFFR/TNFRSF13C ELISA Kit
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Application: For quantitative detection of BAFFR / TNFRSF13C in mouse serum, plasma, body fluids, tissue lysatesor cell culture supernatants.
Introduction: BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C) and BLyS receptor 3 (BR3), is a membrane protein of the TNF receptor superfamily which recognizes BAFF, an essential factor for B cell maturation and survival.In humans it is encoded by the TNFRSF13C gene.B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BAFFR / TNFRSF13C polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BAFFR / TNFRSF13C polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theBAFFR / TNFRSF13Camount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofBAFFR / TNFRSF13Ccan be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 78 pg/ml - 5000 pg/ml
Sensitivity: <20pg/ml
Introduction: BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C) and BLyS receptor 3 (BR3), is a membrane protein of the TNF receptor superfamily which recognizes BAFF, an essential factor for B cell maturation and survival.In humans it is encoded by the TNFRSF13C gene.B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BAFFR / TNFRSF13C polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BAFFR / TNFRSF13C polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theBAFFR / TNFRSF13Camount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofBAFFR / TNFRSF13Ccan be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 78 pg/ml - 5000 pg/ml
Sensitivity: <20pg/ml
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