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Biospes
SKU(재고 관리 코드):BEK1348
Human S100A12/CGRP ELISA Kit
Human S100A12/CGRP ELISA Kit
Application: For quantitative detection of S100A12 / CGRP in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene.Human S100A12, also known as calgranulin C, was first described in 1995.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs.Neutrophils and monocytes / macrophages are important source of S100A12 in the cell although some epithelial cells and dendritic cells are capable of its secretion.[9] Some tissues are rich in these cells, and so in this protein. These include the spleen or lungs. It occurs intracellularly but is also produced into the extracellular environment where it occurs as a homodimer or hexamer.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-S100A12 / CGRP polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-S100A12 / CGRP polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theS100A12 / CGRPamount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofS100A12 / CGRPcan be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: <10 pg/ml
Introduction: S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene.Human S100A12, also known as calgranulin C, was first described in 1995.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs.Neutrophils and monocytes / macrophages are important source of S100A12 in the cell although some epithelial cells and dendritic cells are capable of its secretion.[9] Some tissues are rich in these cells, and so in this protein. These include the spleen or lungs. It occurs intracellularly but is also produced into the extracellular environment where it occurs as a homodimer or hexamer.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-S100A12 / CGRP polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-S100A12 / CGRP polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theS100A12 / CGRPamount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofS100A12 / CGRPcan be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: <10 pg/ml
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