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SKU(재고 관리 코드):LAA571Ra71

Biotin-Linked Polyclonal Antibody to Collagen Type I (COL1)

Biotin-Linked Polyclonal Antibody to Collagen Type I (COL1)

Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.


Organism Species

Rattus norvegicus (Rat).


Antibody labeling

Ig Type



Antigen-specific affinity chromatography followed by Protein A affinity chromatography



Original Antibody


Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.





Organism Species More




If the antibody is used in flow cytometry, please check FCM antibodies.


20µl 100µl 200µl 1ml 10ml


The antibody is a rabbit polyclonal antibody raised against COL1. It has been selected for its ability to recognize COL1 in immunohistochemical staining and western blotting.


Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.


Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.


The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.



Journal of hypertension

Urotensin II induces phenotypic differentiation, migration, and collagen synthesis of adventitial fibroblasts from rat aorta PubMed: 18475149

Cardiovascular Research

Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathway OxfordJournals: source

Acta Otolaryngol

Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa PubMed: 19958244

Experimental Biology and Medicine

Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source

Connect Tissue Res.

Role of moderate exercising on Achilles tendon collagen crimping patterns and proteoglycans Pubmed: 23758268

Bulletin of Experimental Biology and Medicine

Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty Pubmed: 24319714

Age( dordr).

In vitro tenocyte metabolism in aging and oestrogen deficiency Springer: Source


A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source

European Archives of Oto-Rhino-Laryngology

Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats Pubmed: 24077847

Stem Cell Research Therapy

Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658

Physiol. Res.

The unwounded skin remodeling in animal models of diabetes types 1 and 2. Pubmed: 24020818

J Physiol Pharmacol.

Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic diet Pubmed: 23568967


Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses ScienceDirect: S0142961214003135

Journal of Clinical and Experimental Hepatology

Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver Fibrosis ScienceDirect: S0973688314000073

Human Molecular Genetics Online

Dystrophic mdx mice develop severe cardiac and respiratory dysfunction following genetic ablation of the anti-inflammatory cytokine IL-10 Pubmed:24659498

PLoS One.

Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592

Journal of Biomedical Materials Research Part A

Carboxymethyl cellulose—hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications Pubmed:23720392

Experimental Biology adn Medicine

Endostar, a novel human recombinant endostatin, attenuates liver fibrosis in CCl4-induced mice Pubmed:24872431

International Journal of Clinical & Experimental Pathology

Mast cell chymase in keloid induces profibrotic response via transforming growth factor-β1/Smad activation in keloid fibroblasts Pubmed:25120737

FEBS Letters

Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352


KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overload Pubmed:25633973


Impact of the Uremic Milieu on the Osteogenic Potential of Mesenchymal Stem Cells Pubmed:25635832

Journal of Colloid and Interface Science

Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses Pubmed:25706198

Macromolecular Bioscience

Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells PubMed: 26126665

journal of hard Tissue Biology

Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite Article: Jhtb

Mol Med Rep

NIM811 downregulates transforming growth factor PubMed: 26573209

Front Pharmacol

Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats PubMed: 26483688


Effects of fluoride on the ultrastructure and expression of Type I collagen in rat hard tissue PubMed: 25655816

J Surg Res

Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transition PubMed: 25911951

Molecular and Cellular Biochemistry

SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis PubMed: 26112906


Incorporation of nanostructured hydroxyapatite and poly(N-isopropylacrylamide) in demineralized bone matrix enhances osteoblast and human mesenchymal stem cell activity PubMed: 26443012


On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery 2310-2861: 1

Acta Biomater

Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study PubMed: 26689470

Int J Clin Exp Med

Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … PubMed: 26309506

International Journal of Molecular Sciences

Hybrid Macro-Porous Titanium Ornamented by Degradable 3D Gel/nHA Micro-Scaffolds for Bone Tissue Regeneration Pubmed:27092492


白细胞介素10 上调表达对家兔慢性细菌性鼻窦炎黏膜创伤修复的影响 article:11593

Acta Biomaterialia

Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study Pubmed:26689470

scientific reports

IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy Pubmed:27263531

Cell Mol Immunol 

Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496


Інфаркт міокарда, ускладнений аневризмою лівого шлуночка: клінічні і структурно-функціональні особливості перебігу, діагностичні, терапевтичні та прогностичні аспекти bitstream:123456789

Inflammation Research

Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy pubmed:27516211

Indian Journal of Pharmaceutical Education and Research

Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF-β1 sites:default

Heart & Vessels

Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR pubmed:28004175

biochemical and biophysical research communications

Positive feedback loop of YB-1 interacting with Smad2 promotes liver fibrosis. pubmed:28153731

Frontiers in Pharmacology

Induced pluripotent stem cells inhibit bleomycin-induced pulmonary fibrosis in mice through suppressing TGF-β1/Smad-mediated epithelial to mesenchymal … PMC5108931

International Journal of Molecular Sciences

Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers'Pneumoconiosis: A Case-Control Study. pubmed:27556453

BMC Cancer

Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells. pubmed:27461108

J Cell Physiol.

An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. pubmed:28240358

Basic Clin Pharmacol Toxicol.

Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440

Acta Biomater.

Antiresorptive and anti-angiogenetic octacalcium phosphate functionalized with bisphosphonates: An in vitro tri-culture study. pubmed:28238916

The American Journal of Chinese Medicine

Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells pubmed:28231747

Biomedical and Environmental Sciences

Crystalline Silica Promotes Rat Fibrocyte Differentiation in Vitro, and Fibrocytes Participate in Silicosis in Vivo pubmed:29081339

Basic & Clinical Pharmacology & Toxicology

Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling doi:10.1111

Dental Materials Journal

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells. pubmed:28228629

Biomedicine & pharmacotherapy

Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995

Journal of Applied Biomaterials & Functional Materials

Demineralized dentin and enamel matrices as suitable substrates for bone regeneration pubmed:28731486

Acta Biomaterialia

Selective effect of hydroxyapatite nanoparticles on osteoporotic and healthy bone formation correlates with intracellular calcium homeostasis regulation pubmed:28698163

Advanced Healthcare Materials

Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. pubmed:29120858

Macromolecular Bioscience

Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response Pubmed:29877029

International Journal of Molecular Medicine

Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced … Pubmed:29620144

PLoS One

Effect of hyperbaric oxygen therapy (HBO) on implant-associated osteitis in a femur fracture model in mice Pubmed:29377928

Royal Society of Chemistry

In vitro and in vivo assessment of nanostructured porous biphasic calcium phosphate ceramics for promoting osteogenesis in an osteoporotic environment c8ra00768c

European Food Research and Technology

Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis 10.1007:s00217-018-3119-z

Journal of Applied Biomaterials & Functional Materials

BMP-2 and type I collagen preservation in human deciduous teeth after demineralization Pubmed:30045659


Effect of transforming growth factor-β1 on α-smooth muscle actin and collagen expression in equine endometrial fibroblasts Pubmed: 30321755

ACS Applied Materials & Interfaces

Fabrication and characterization of core-shell nanofibers using a next-generation airbrush for biomedical applications Pubmed: 30433758

Chemico-Biological Interactions

Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells Pubmed: 30731080

Oxidative Medicine and Cellular Longevity

Effects of the (Pro) renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway

Tropical Journal of Pharmaceutical Research

Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats.

Scientific Reports

Low fibrosis biomarker levels predict cardiac resynchronization therapy response Pubmed: 30988339

Experimental Lung Research

High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects Pubmed: 31290711

BMC Nephrology

Serum sclerostin levels are positively related to bone mineral density in peritoneal dialysis patients: a cross-sectional study Pubmed: 31315601

Archives of Dermatological Research

Extracellular matrix deposition by adipose-derived stem cells and fibroblasts: a comparative study Pubmed: 31616972

Drug Delivery and Translational Research

A multifunctional dexamethasone-delivery implant fabricated using atmospheric plasma and its effects on apoptosis, osteogenesis and inflammation Pubmed: 31898081


Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs Pubmed: 32146192

Molecular Metabolism

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α Pubmed: 32029232

ACS Applied Materials & Interfaces

ZIF-8 Modified Multifunctional Bone-Adhesive Hydrogels Promoting Angiogenesis and Osteogenesis for Bone Regeneration Pubmed: 32814397

in vitro cellular & developmental biology-animal

Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix Pubmed: 32588253

Molecular Medicine

Circulating fibrocytes traffic to the lung in murine acute lung injury and predict outcomes in human acute respiratory distress syndrome: a pilot study Pubmed: 32460694

Predictors of myocardial fibrosis and loss of epicardial adipose tissue volume in the long-term period after myocardial infarction

ACS Biomaterials Science & Engineering

Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction

Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда

Ann Med Surg

Trauma magnitude of the meta-epyphyseal cancellous affects the incidence of deep vein thrombosis. A prospective cohort study on the dynamic of collagen I ¡­ 33717478


Uncarboxylated osteocalcin reverses the high glucose?induced inhibition of the osteogenic differentiation of MC3T3E1 cells via the GPRC6A/cAMP/PKA/AMPK signaling pathway 33786607

PLoS One

Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies 33999928

Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells

Russian Journal of Cardiology

Early diagnosis of myocardial fibrosis in patients with epicardial obesity

Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction

Parasit Vectors

Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate … 34857049

Pharmacol Res

Esomeprazole Alleviates Fibrosis in Systemic Sclerosis by Modulating AhR/Smad2/3 Signaling Pubmed:34995795

Catalog No.

Related products for research use of Rattus norvegicus (Rat) Organism species

Applications (RESEARCH USE ONLY!)


Native Collagen Type I (COL1)

Positive Control; Immunogen; SDS-PAGE; WB.


Polyclonal Antibody to Collagen Type I (COL1)



Biotin-Linked Polyclonal Antibody to Collagen Type I (COL1)



ELISA Kit for Collagen Type I (COL1)

Enzyme-linked immunosorbent assay for Antigen Detection.


Magnetic Luminex Assay Kit for Collagen Type I (COL1) ,etc.

Magnetic Luminex Assay for Antigen Detection.


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