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SKU(재고 관리 코드):LAA028Hu71

Biotin-Linked Polyclonal Antibody to Erythropoietin (EPO)

Biotin-Linked Polyclonal Antibody to Erythropoietin (EPO)

UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.

Product No.

LAA028Hu71

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

PAA028Hu01-Polyclonal Antibody to Erythropoietin (EPO)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against EPO. It has been selected for its ability to recognize EPO in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Endocrinology

Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring Endo: source

Journal of Ethnopharmacology

Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression PubMed: 21333724

PLOS ONE

CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation PlosOne: Source

The Journal of Clinical Endocrinology & Metabolism

Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation PubMed: 22948762

Experimental & Molecular Medicine

Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression PubMed: PMC3509184

Biochem Biophys Res Commun.

Reevaluation of erythropoietin production by the nephron Pubmed:24832733

Nutrition.

Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. Pubmed:2528040

J Appl Physiol (1985).

Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness Pubmed:25324511

Expert Rev Proteomics

Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone PubMed: 26496240

Oncotarget

KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin pubmed:26575329

international journal of molecular sciences

Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. pubmed:28216608

Veterinary Clinical Pathology

Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease pubmed:28186645

IOS Press Content Library

An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease

Oxidative Medicine and Cellular Longevity

Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation pubmed:29147463

PLoS Neglected Tropical Diseases

Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis pubmed:28489854

Neurochemistry International

Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia S0197018617304783

Pesquisa Veterinária Brasileira

Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease 10.1590/s0100-736x2017000600011 

Medical Journal Armed Forces India

Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation 10.1016/j.mjafi.2017.04.003

Neurochemistry International

Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on … Pubmed:29180303

Clinical and Experimental Medicine

Increased NGAL level associated with iron store in chronic kidney disease with anemia Pubmed:29909502

Bulletin of Experimental Biology and Medicine

Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia Pubmed:29308565

Bulletin of Experimental Biology and Medicine

Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … Pubmed: 30353332

FASEB JOURNAL

High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans

Journal of Functional Foods

Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats

博士论文

PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE

Nutrition

Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind … Pubmed: 31743809

BRITISH JOURNAL OF HAEMATOLOGY

Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans Pubmed: 32866306

JCI Insight.

Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes Pubmed: 32759495

Blood Cells, Molecules, and Diseases

EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness Pubmed: 32470757

Int J Endocrinol

miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia 34367277

J Mater Sci Mater Med

Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules Pubmed:35072831


Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells


Catalog No.

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Applications (RESEARCH USE ONLY!)

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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Monoclonal Antibody to Erythropoietin (EPO)

WB; IHC; ICC; IP.

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Monoclonal Antibody to Erythropoietin (EPO)

WB,IHC

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ELISA Kit for Erythropoietin (EPO)

Enzyme-linked immunosorbent assay for Antigen Detection.

SCA028Hu

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Chemiluminescent immunoassay for Antigen Detection.

LMA028Hu

Magnetic Luminex Assay Kit for Erythropoietin (EPO) ,etc.

Magnetic Luminex Assay for Antigen Detection.

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ELISA Kit DIY Materials for Erythropoietin (EPO)

Main materials for "Do It (ELISA Kit) Yourself".

 

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