BLK; Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human BLK. AA range:456-505
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
58kD
GeneID（Human）:
640
Human Swiss-Prot No:
P51451
Cellular localization:
Cell membrane ; Lipid-anchor . Present and active in lipid rafts. Membrane location is required for the phosphorylation of CD79A and CD79B (By similarity). .
Background:
This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010],
Western Blot analysis of various cells using Blk Polyclonal Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).