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ELK Biotechnology
SKU(재고 관리 코드):ES7812
CaMKIα (phospho Thr177) Rabbit Polyclonal Antibody
CaMKIα (phospho Thr177) Rabbit Polyclonal Antibody
CaMKIα (phospho Thr177) Rabbit Polyclonal Antibody
Overview
| Product name: | CaMKIα (phospho Thr177) rabbit pAb |
| Alternative Names: | CAMK1; Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alpha |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. AA range:143-192 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 41kD |
| GeneID(Human): | 8536 |
| Human Swiss-Prot No: | Q14012 |
| Cellular localization: | Cytoplasm . Nucleus . Predominantly cytoplasmic. . |
| Background: | Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK1-alpha (Phospho-Thr177) Antibody -
Immunofluorescence analysis of HeLa cells, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15' and Jurkat cells treated with insulin 0.01U/ml 15', using CaMK1-alpha (Phospho-Thr177) Antibody. The lane on the right is blocked with the phospho peptide.
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