PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality:
Monoclonal
Isotype:
IgG1
Concentration:
1mg/ml
Observed band:
47（51）kDa
GeneID（Human）:
842
Human Swiss-Prot No:
P55211
Cellular localization:
Cytosol,Mitochondrion,Nucleus
Background:
Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Western blot analysis of Hela with Caspase 9 Mouse mAb diluted at 1）1:2,000 2）1:5,000
Western blot analysis of 1）Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 Mouse mAb diluted at 1:3,000
1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1077 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
IF analysis of Hela with EM1077(Left) and DAPI (Right) diluted at 1:100.