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ELK Biotechnology

ELK9742

Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

Sensitivity

Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

Overview

Product name: Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
Reactivity: Cattle
Alternative Names: NT-Pro-ANP; N-ANP
Assay Type: Sandwich
Sensitivity: 0.251 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
40.00 2.291 2.196
20.00 1.587 1.492
10.00 1.282 1.187
5.00 0.933 0.838
2.50 0.521 0.426
1.25 0.357 0.262
0.63 0.219 0.124
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProANP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProANP in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 89-103% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-99% 85-101% 78-97%
EDTA plasma(n=5) 86-97% 81-103% 88-97% 89-104%
Heparin plasma(n=5) 97-106% 93-101% 85-98% 90-101%
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