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ELK Biotechnology

ELK9694

Cattle sRAGE(Soluble receptor for advanced glycation endproducts) ELISA Kit

Cattle sRAGE(Soluble receptor for advanced glycation endproducts) ELISA Kit

Sensitivity

Cattle sRAGE(Soluble receptor for advanced glycation endproducts) ELISA Kit

Overview

Product name: Cattle sRAGE(Soluble receptor for advanced glycation endproducts) ELISA Kit
Reactivity: Cattle
Alternative Names: sRAGE
Assay Type: Sandwich
Sensitivity: 3.93 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle sRAGE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle sRAGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle sRAGE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle sRAGE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
800.00 1.941 1.856
400.00 1.638 1.553
200.00 1.188 1.103
100.00 0.921 0.836
50.00 0.547 0.462
25.00 0.379 0.294
12.50 0.202 0.117
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sRAGE and the recovery rates were calculated by comparing the measured value to the expected amount of sRAGE in samples.
Matrix Recovery range Average
serum(n=5) 96-107% 102%
EDTA plasma(n=5) 80-92% 86%
Heparin plasma(n=5) 88-103% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sRAGE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-106% 83-98% 93-105% 95-104%
EDTA plasma(n=5) 97-106% 85-98% 97-106% 86-92%
Heparin plasma(n=5) 91-103% 89-97% 85-94% 97-106%
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