Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human CD9. AA range:101-150
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
25kD
GeneID（Human）:
928
Human Swiss-Prot No:
P21926
Cellular localization:
Cell membrane ; Multi-pass membrane protein . Membrane ; Multi-pass membrane protein . Secreted, extracellular exosome . Present at the cell surface of oocytes. Accumulates in the adhesion area between the sperm and egg following interaction between IZUMO1 and its receptor IZUMO1R/JUNO. .
Background:
This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011],
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western Blot analysis of various cells using CD9 Polyclonal Antibody diluted at 1:1000
Western Blot analysis of 3T3 cells using CD9 Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of HeLa cells, using CD9 Antibody. The picture on the right is blocked with the synthesized peptide.