Chk1 Polyclonal Antibody

Checkpoint kinase 1encoded by CHEK1 belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for CHEK1.

The CHK1 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of CHK1 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the N-terminal region of human CHK1 protein. The resulting polyclonal antibody is purified using protein A affinity chromatography to ensure high specificity and purity.

The CHK1 Polyclonal Antibody is suitable for use in a variety of applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been validated for use in human, mouse, and rat samples, and has been shown to detect endogenous CHK1 protein in a variety of cell lines.

This antibody is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. It is recommended to store the antibody at -20°C for long-term storage and to avoid repeated freeze-thaw cycles.

Overall, the CHK1 Polyclonal Antibody is a reliable and high-quality tool for the detection of CHK1 protein in various biological samples, making it an essential component of any research project focused on this important protein.