Cleaved-COL4A3 (L1425) Polyclonal Antibody

Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. COL4A3 encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli.

The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. COL4A3 is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region.

Like the other members of the type IV collagen gene family, COL4A3 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.

The Cleaved-COL4A3 (L1425) Polyclonal Antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of cleaved collagen type IV alpha 3 (COL4A3) protein in various biological samples.

This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the cleavage site of COL4A3 protein, and subsequently purified using protein A affinity chromatography. The antibody has been validated for use in various applications, including Western blotting, immunohistochemistry, and ELISA. It exhibits high affinity and specificity towards the cleaved form of COL4A3 protein, and does not cross-react with other related proteins.

The Cleaved-COL4A3 (L1425) Polyclonal Antibody is an essential tool for researchers studying the role of COL4A3 protein in various physiological and pathological processes, including cancer, inflammation, and fibrosis. Its high sensitivity and specificity make it an ideal reagent for accurate and reliable detection and quantification of cleaved COL4A3 protein in biological samples.