Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human CtBP1. AA range:388-437
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
48kD
GeneID（Human）:
1487
Human Swiss-Prot No:
Q13363
Cellular localization:
Cytoplasm . Nucleus .
Background:
This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008],
Western Blot analysis of Jurkat cells using CtBP1 Polyclonal Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CtBP1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using CtBP1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night