Cusabio
SKU(재고 관리 코드):CSB-MA000011M1m
His Monoclonal Antibody
His Monoclonal Antibody
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| Release Date | 2023-12-13 |
|---|---|
| Product Code | CSB-MA000011M1m |
| Short Name | His Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ul, $120/50ul |
| Species Reactivity | Species Independent |
| Tested Applications | ELISA, WB, IP |
| Dilution | WB:1:1000-1:640000, IP:2µl-5µl |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | The immunogen is a mixture of various His-tagged proteins |
| Antigen Species | Species Independent |
| Target Name | His Tag |
| Clonality | Monoclonal |
| Clone ID | 2F4B5 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12935188/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: 200ng recombinant protein with His tag
His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4 KDa
Observed band size: 43.4 KDa
Exposure time：5min
Image 2
Western Blot
Positive WB detected in: 200ng recombinant protein with His tag
His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size:62.5 KDa
Observed band size: 66 KDa
Exposure time：5min
Image 3
Western Blot
Positive WB detected in:200ng of four Recombinant protein with his tag
All lanes: His antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4, 62.5,56,80.6 KDa
Observed band size:43.4, 66, 57 ,90 KDa
Exposure time：5min
Image 4
Immunoprecipitating His in 293 whole cell lysate transfected with His
Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His.
Lane 2: 2F4B5 antibody(2µg) + 293 whole cell lysate transfected with His (500µg)
Lane 3: 293 whole cell lysate transfected with His (5µg)
For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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