Cusabio
SKU(재고 관리 코드):CSB-MA000041M0m
Myc tag Monoclonal antibody
Myc tag Monoclonal antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA000041M0m |
| Short Name | Myc tag Monoclonal antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ug, $120/50ug |
| Species Reactivity | Species Independent |
| Tested Applications | ELISA, WB, IF, IP |
| Dilution | WB:1:500-1:5000, IF:1:100-1:300, IP:1:1000-1:1500 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | EQKLISEEDL (Myc) synthetic peptide conjugate to KLH |
| Antigen Species | Species Independent |
| Target Name | Myc tag |
| Clonality | Monoclonal |
| Clone ID | 5F92F9 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/1028796/ |
| Size | 100ug |
Image 1
WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
Lane 1: Recombinant Myc-tagged fusion protein at 50ng
Lane 2: Recombinant Myc-tagged fusion protein at 25ng
Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 50 kd
Observed band size: 50 kd
Image 2
Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 3
Immunoprecipitating MYC-tag with transfected HEK293
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)
Lane 3: Transfected HEK293 whole cell lysate (10µg)
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