Cusabio
SKU(재고 관리 코드):CSB-MA000091M1m
ACTB Monoclonal Antibody
ACTB Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA000091M1m |
| Short Name | ACTB Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ul, $120/50ul |
| Species Reactivity | Human, Mouse, Rat, Rabbit |
| Tested Applications | ELISA, WB, IHC, IF, FC |
| Dilution | WB:1:5000-1:80000, IHC:1:500-1:1000, IF:1:50-1:200, FC:1:100-1:300 |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | A synthesized peptide derived from human Beta-Actin (1-50aa) |
| Antigen Species | Human |
| Uniprot ID | P60709 |
| Target Name | ACTB |
| Clonality | Monoclonal |
| Clone ID | 1D12E12B5 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12930731/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Image 2
Western Blot
Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, Rabbit kidney tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Image 3
Western Blot
Positive WB detected in: Mouse liver tissue, Mouse brain tissue, Mouse lung tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse stomach tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Image 4
Western Blot
Positive WB detected in: Rabbit liver tissue, Rabbit brain tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit stomach tissue, A549 whole cell lysate
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Image 5
Western Blot
Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, U87 whole cell lysate
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Image 6
Western Blot
Positive WB detected in: 293T whole cell lysate at 40μg, 20μg, 10μg
All lanes:ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Image 7
Western Blot
Positive WB detected in: 20μg 293T whole cell lysate
ACTB antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Image 8
IHC image of CSB-MA000091M1m diluted at 1:500 and staining in paraffin-embedded colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37鈩?Then primary antibody (1% BSA) was incubated at 4掳C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Image 9
IHC image of CSB-MA000091M1m diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37鈩?Then primary antibody (1% BSA) was incubated at 4掳C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Image 10
Immunofluorescence staining of Hela cells with CSB-MA000091M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4掳C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG 锛圚+L锛?
Image 11
Immunofluorescence staining of HepG2 cells with CSB-MA000091M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4掳C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG 锛圚+L锛?
Image 12
Overlay Peak curve showing Hela cells stained with CSB-MA000091M1m (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1碌g/1*106cells) for 1 h at 4掳C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4掳C. Isotype control antibody (green line) was mouse IgG2b (1碌g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Image 13
Overlay Peak curve showing HepG2 cells stained with CSB-MA000091M1m (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1碌g/1*106cells) for 1 h at 4掳C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4掳C. Isotype control antibody (green line) was mouse IgG2b (1碌g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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