Cusabio
SKU(재고 관리 코드):CSB-MA000091M2m
ACTB Monoclonal Antibody
ACTB Monoclonal Antibody
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| Release Date | 2023-01-18 |
|---|---|
| Product Code | CSB-MA000091M2m |
| Short Name | ACTB Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ul, $120/50ul |
| Species Reactivity | Human, Mouse, Rat, Rabbit |
| Tested Applications | ELISA, WB |
| Dilution | WB:1:10000-1:1280000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | A synthesized peptide derived from human Beta-Actin (1-50aa) |
| Antigen Species | Human |
| Uniprot ID | P60709 |
| Target Name | ACTB |
| Clonality | Monoclonal |
| Clone ID | 6D8D8 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12932123/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue, Rat heart tissue, Rat skeletal muscle tissue, Rabbit lung tissue, Rabbit liver tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Image 2
Western Blot
Positive WB detected in: 293T whole cell lysate, Zebrafish tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Image 3
Western Blot
Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse skeletal muscle tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Image 4
Western Blot
Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat kidney tissue, U87 whole cell lysate Rat skeletal muscle tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Image 5
Western Blot
Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit lung tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue, A549 whole cell lysate, MCF-7 whole cell lysate
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Image 6
Western Blot
Positive WB detected in: Hela whole cell lysate at 40μg, 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Image 7
Western Blot
Positive WB detected in: 20μg Hela whole cell lysate
ACTB antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
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