Cusabio
SKU(재고 관리 코드):CSB-MA000101M0m
Avi-Tag Monoclonal Antibody
Avi-Tag Monoclonal Antibody
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| Release Date | 2023-01-18 |
|---|---|
| Product Code | CSB-MA000101M0m |
| Short Name | Avi-Tag Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ul, $120/50ul |
| Species Reactivity | Species Independent |
| Tested Applications | ELISA, WB, IP |
| Dilution | WB:1:1000-1:640000, IP:2µl-5µl |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Synthetic Peptide |
| Antigen Species | Species Independent |
| Target Name | AVI |
| Clonality | Monoclonal |
| Clone ID | 9G3B5 |
| Host Species | Mouse |
| Isotype | IgG2a |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12931934/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli and Mammalian cell
All lanes: Avi tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40, 35, 30, 70, 40, 33, 35, 40 KDa
Observed band size: 40, 35, 30, 70, 40, 33, 35, 4 KDa
Exposure time：1min
Image 2
Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli
All lanes: Avi tag antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 81.2, 67, 66.9, 66.9 KDa
Observed band size: 82, 67, 67, 67KDa
Exposure time：5min
Image 3
Western Blot
Positive WB detected in: Recombinant protein from Mammalian cell with Avi tag from E.coli at 100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
All lanes: Avi tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40 KDa
Observed band size: 40 KDa
Exposure time：1min
Image 4
Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from Mammalian cell
Avi tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000, 1:12800000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40 KDa
Observed band size: 40 KDa
Exposure time：5min
Image 5
Immunoprecipitating Avi tag in 293F whole cell lysate transfected with avi tag
Lane 1: Mouse control IgG instead of CSB-MA000101M0m in 293F whole cell lysate transfected with avi tag.
Lane 2: CSB-MA000101M0m (1µg) + 293F whole cell lysate transfected with avi tag (500µg)
Lane 3: 293F whole cell lysate transfected with avi tag(40µg)
For western blotting, the blot was detected with CSB-MA000101M0m at 1:1000, and a HRP-conjugated Goat anti Mouse antibody was used as the secondary antibody at 1:50000
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