Cusabio
SKU(재고 관리 코드):CSB-MA000141M0m
HA-Tag Monoclonal Antibody
HA-Tag Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA000141M0m |
| Short Name | HA-Tag Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ul, $120/50ul |
| Species Reactivity | Species Independent |
| Tested Applications | ELISA, WB, IF, FC, IP |
| Dilution | WB:1:5000-1:160000, IF:1:50-1:200, IP:1:5000-1:100000, FC：1:50-1:200 |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | YPYDVPDYA synthetic peptide conjugate to KLH |
| Antigen Species | Species Independent |
| Target Name | HA-Tag |
| Clonality | Monoclonal |
| Clone ID | 18B11H6 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12927411/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: 3 different overexpression lysates with HA tagged
All lanes: HA-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 35, 48 kDa
Observed band size: 35, 35, 48 kDa</p>
Image 2
Western Blot
Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
All lanes: HA-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa</p>
Image 3
Western Blot
Positive WB detected in: HA-tagged fusion protein
All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa</p>
Image 4
Western Blot
Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
All lanes: HA-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution</p>
Image 5
Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 6
Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 7
Immunofluorescence staining of 293F cells and 293F transfected cells with Company A, Company B, Company C, Company D, Company E, CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 8
Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg)
Image 9
Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: Company A (5µg), Company B (5µg),Company C (5µg),Company D (5µg),Company E (5µg),CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg)
Image 10
Overlay histogram showing 293F transfected whole cell stained with CSB-MA000141M0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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