Cusabio
SKU(재고 관리 코드):CSB-MA000151M0m
E-Tag Monoclonal Antibody
E-Tag Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA000151M0m |
| Short Name | E-Tag Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ul, $120/50ul |
| Species Reactivity | Species Independent |
| Tested Applications | ELISA, WB, IP |
| Dilution | ELISA:1:4000-1:8000, WB:1:1000-1:320000, IP:1µg-5µg |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | GAPVPYPDPLEPRC synthetic peptide conjugate to KLH |
| Antigen Species | Species Independent |
| Target Name | E-Tag |
| Clonality | Monoclonal |
| Clone ID | 11H12B3 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12927526/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
All lanes: E-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Image 2
Western Blot
Positive WB detected in: E-tagged fusion protein
All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Image 3
Immunoprecipitating E-Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg)
Image 4
ELISA E-Tag antibody in E-tagged fusion protein and Control protein(no E-tag)
Antigen at dilution of 1ug/ml
E-Tag antibody at (0-0.15ug)
Goat polyclonal to Mouse IgG at 1/50000 dilution
Image 5
Western Blot
Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
All lanes: E-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 30, 68, 55, 67-157kDa
Observed band size: 30, 68, 55, 67-157kDa
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