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Cusabio

SKU(재고 관리 코드):CSB-MA000158

HA-Tag Monoclonal Antibody

HA-Tag Monoclonal Antibody

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-MA000158
Short Name HA-Tag Monoclonal Antibody
Antibody Type Monoclonal Antibody
List Price $199/100ug, $119/50ug
Species Reactivity Species Independent
Tested Applications ELISA, WB, IF, IP
Dilution WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000
Purification Method Affinity chromatography
Buffer Formulation PBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium Azide, pH 7.4.
Antigen Synthetic Peptide
Antigen Species Species Independent
Target Name HA-Tag
Clonality Monoclonal
Clone ID 26E2
Host Species Mouse
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/11090317/
Size 50ug

Image 1

IP antibody use: 5µg HA Mouse IgG1 per ml Lysate, WB 1:5000

Lane 1: untransfected 293 cell lysate

Lane 2: transfected 293 cell lysate with HA-tag fusion protein

Lane 3: IP (untransfted 293 + anti-HA mAb + Protein G agarose)

Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)

Lane 5: IP (transfected 293 + anti-HA mAb + Protein G agarose)

Lane 6: IP (transfected 293 + Protein G)

Lane 7: Recombinant protein (E.coli)

Image 2

IF analysis of 293 cells transfected with a HA-tag protein,1:2000 dilution (blue DAPI, red anti-HA)

Image 3

0.5µg HA fusion protein + Primary antibody dilution at 1:10000

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