Cusabio
SKU(재고 관리 코드):CSB-MA000161M0m
V5-Tag Monoclonal Antibody
V5-Tag Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA000161M0m |
| Short Name | V5-Tag Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ul, $120/50ul |
| Species Reactivity | Species Independent |
| Tested Applications | ELISA, WB, IF, IP, FC |
| Dilution | WB:1:2000-1:80000, IF:1:100-1:200, IP:2µl-5µl, FC:1:100-1:300 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | GKPIPNPLLGLDST synthetic peptide conjugate to KLH |
| Antigen Species | Species Independent |
| Target Name | V5-Tag |
| Clonality | Monoclonal |
| Clone ID | 8A7A8 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12931894/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: 50ng V5-tagged fusion protein
V5 Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 37.8 KDa
Observed band size: 43 KDa
Exposure time: 10S
Image 2
Western Blot
Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:5000
Lane 1: Recombinant V5-tagged fusion protein at 50ng
Lane 2: Recombinant V5-tagged fusion protein at 25ng
Lane 3: Recombinant V5-tagged fusion protein at 12.25ng
Lane 4: Recombinant V5-tagged fusion protein at 6.25ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 37.8 kd
Observed band size: 43 kd
Exposure time: 5min
Image 3
Western Blot
Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4 at 50ng
All lanes: V5 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50.2, 47.6, 66.7, 37.8 KDa
Observed band size: 52, 47, 67, 42 KDa
Exposure time: 30s
Image 4
Western Blot
Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, at 50ng
All lanes: V5 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31.9, 20.4, 24.2, 53.4 KDa
Observed band size: 32, 21, 28, 58 KDa
Exposure time: 5min
Image 5
Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).
Image 6
Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
Lane 1: Mouse control IgG1 instead of CSB-MA000161M0m in Transfected HEK293F cells whole cell lysate
Lane 2: CSB-MA000161M0m (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg)
Lane 3: Transfected HEK293F cells whole cell lysate (20µg)
For western blotting, the blot was detected with CSB-MA000161M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
Image 7
Overlay histogram showing untransfected HEK293F cells (green line) or transfected HEK293F cells (red line) stained with CSB-MA000161M0m. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*10<sup>6</sup> cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Acquisition of >10,000 events was performed.
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