Cusabio
SKU(재고 관리 코드):CSB-MA000161M1m
V5-Tag Monoclonal Antibody
V5-Tag Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA000161M1m |
| Short Name | V5-Tag Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ul, $120/50ul |
| Species Reactivity | Species Independent |
| Tested Applications | ELISA, WB, IF, IP |
| Dilution | WB:1:5000-1:1280000, IF:1:100-1:200, IP:2µl-5µl |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | GKPIPNPLLGLDST synthetic peptide conjugate to KLH |
| Antigen Species | Species Independent |
| Target Name | V5-Tag |
| Clonality | Monoclonal |
| Clone ID | 9E8B7 |
| Host Species | Mouse |
| Isotype | IgG2a |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12931895/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, 5, 6 at 50ng
All lanes: V5 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31.9, 20.4, 55, 24.2, 53.4, 30 KDa
Observed band size: 32, 21, 55, 28, 58, 33 KDa
Exposure time: 5min
Image 2
Western Blot
Positive WB detected in: 50ng V5-tagged fusion protein
V5 Tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 KDa
Exposure time: 5min
Image 3
Western Blot
Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:2000
Lane 1: Recombinant V5-tagged fusion protein at 25ng
Lane 2: Recombinant V5-tagged fusion protein at 12.5ng
Lane 3: Recombinant V5-tagged fusion protein at 6.25ng
Lane 4: Recombinant V5-tagged fusion protein at 3.125ng
Lane 5: Recombinant V5-tagged fusion protein at 1.56ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 32 kd
Observed band size: 32 kd
Exposure time: 5min
Image 4
Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).
Image 5
Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
Lane 1: Mouse control IgG2a instead of CSB-MA000161M1m in Transfected HEK293 cells whole cell lysate
Lane 2: CSB-MA000161M1m (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg)
Lane 3: Transfected HEK293F cells whole cell lysate (20µg)
For western blotting, the blot was detected with CSB-MA000161M1m at 1:3000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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