Cusabio
SKU(재고 관리 코드):CSB-MA004888A0m
CD19 Monoclonal Antibody
CD19 Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA004888A0m |
| Short Name | CD19 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF, FC |
| Dilution | WB:1:2000-1:80000, IHC:1:50-1:200, IF:1:50-1:200 |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human CD19 protein (20-291aa) |
| Antigen Species | Human |
| Uniprot ID | P15391 |
| Target Name | CD19 |
| Clonality | Monoclonal |
| Clone ID | 8G11B7 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12925748/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: CD19 antibody at 1:2000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 95 kDa
Image 2
Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: CD19 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 95 kDa
Image 3
IHC image of CSB-MA004888A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 4
IHC image of CSB-MA004888A0m diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 5
Immunofluorescence staining of Hela cells with CSB-MA004888A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 6
Immunofluorescence staining of Raji cells with CSB-MA004888A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 7
Overlay histogram showing Raji cells stained with CSB-MA004888A0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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