Cusabio
SKU(재고 관리 코드):CSB-MA004969A0m
CD9 Monoclonal Antibody
CD9 Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA004969A0m |
| Short Name | CD9 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:2000-1:16000, IHC:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human CD9 antigen protein (112-195aa) |
| Antigen Species | Human |
| Uniprot ID | P21926 |
| Target Name | CD9 |
| Clonality | Monoclonal |
| Clone ID | 5A6D8 |
| Host Species | Mouse |
| Isotype | IgG2a |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12928588/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate
All lanes CD9 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time：5min
Image 2
Western Blot
Positive WB detected in: A549 whole cell lysate at 20μg, 10μg, 5μg, 2.5μg
All lanes: CD9 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time：10min
Image 3
Western Blot
Positive WB detected in: 20μg A549 whole cell lysate
CD9 antibody at 1:2000, 1:4000, 1:8000, 1:16000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time：10min
Image 4
Western Blot
Positive WB detected in:
1. Exosomes extracted from plasma
2. Exosomes extracted from serum
3. Exosomes extracted from urine
4. Exosomes extracted in cell supernatant
5. Exosomes extracted from latex
6. Exosomes extracted from saliva
7. Hela whole cell lysate
All lanes CD9 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time：5min
Image 5
Western Blot
Positive WB detected in:
1. Exosomes extracted from saliva
2. Hela whole cell lysate
All lanes Ywhaz antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time：5min
Image 6
IHC image of MA004969A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Image 7
IHC image of MA004969A0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
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