Cusabio
SKU(재고 관리 코드):CSB-MA006327A0m
CYC1 Monoclonal Antibody
CYC1 Monoclonal Antibody
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| Release Date | 2023-01-18 |
|---|---|
| Product Code | CSB-MA006327A0m |
| Short Name | CYC1 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, FC |
| Dilution | WB:1:1000-1:5000, FC:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Cytochrome c1 protein (85-325aa) |
| Antigen Species | Human |
| Uniprot ID | P08574 |
| Target Name | CYC1 |
| Clonality | Monoclonal |
| Clone ID | 27E12D11 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12932101/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: CYC1 antibody at 1:1000
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: U251 whole cell lysate
Lane 6: A549 whole cell lysate
Lane 7: NIH/3T3 whole cell lysate
Lane 8: PC-3 whole cell lysate
Secondary
Goat polyclonal to Mouse IgG at 1/20000 dilution
Predicted band size: 35 KDa
Observed band size: 35 KDa
Exposure time: 5min
Image 2
Overlay Peak curve showing HepG2 cells stained with CSB-MA006327A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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