Cusabio
SKU(재고 관리 코드):CSB-MA008727A0m
FLOT1 Monoclonal Antibody
FLOT1 Monoclonal Antibody
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| Release Date | 2023-01-18 |
|---|---|
| Product Code | CSB-MA008727A0m |
| Short Name | FLOT1 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IF, FC |
| Dilution | WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant FLOT1 protein (149-427aa) |
| Antigen Species | Human |
| Uniprot ID | O75955 |
| Target Name | FLOT1 |
| Clonality | Monoclonal |
| Clone ID | 4C8B6 |
| Host Species | Mouse |
| Isotype | IgG2a |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12931932/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, 3T3 whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue
All lanes: FLOT1 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 48, 43 kDa
Observed band size: 48 KDa
Exposure time：5min
Image 2
IHC image of CSB-MA008727A0m diluted at 1:100 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 3
IHC image of CSB-MA008727A0m diluted at 1:100 and staining in paraffin-embedded human cervical cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 4
Immunofluorescence staining of Hela cells with CSB-MA008727A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 5
Overlay Peak curve showing Hela cells stained with CSB-MA008727A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup> cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10<sup>6</sup> cells) used under the same conditions. Acquisition of >10,000 events was performed.
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