Cusabio
SKU(재고 관리 코드):CSB-MA009476A0m
lacZ Monoclonal Antibody
lacZ Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA009476A0m |
| Short Name | lacZ Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Escherichia coli |
| Tested Applications | ELISA, WB, IF, IP, FC |
| Dilution | WB:1:1000-1:64000, IF:1:50-1:200, IP:1ul-4ul, FC:1:100-1:300 |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant Escherichia coli Beta-galactosidase protein |
| Antigen Species | Escherichia coli |
| Uniprot ID | P00722 |
| Target Name | lacZ |
| Clonality | Monoclonal |
| Clone ID | 3B7D5 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12925751/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
All lanes: lacZ antibody at 1:2000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 117 kDa
Observed band size: 130 kDa
Image 2
Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
Image 3
Immunoprecipitating lacZ in 293T transfected whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysate.
Lane 2: CSB-MA009476A0m (2µl) + 293T transfected whole cell lysate (500µg)
Lane 3: 293T transfected whole cell lysate (20µg)
For western blotting, the blot was detected with CSB-MA009476A0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
Image 4
Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.
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