Cusabio
SKU(재고 관리 코드):CSB-MA010829A0m
HSPA8 Monoclonal Antibody
HSPA8 Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA010829A0m |
| Short Name | HSPA8 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Rat, Mouse |
| Tested Applications | ELISA, WB, IHC, FC, IP |
| Dilution | WB:1:10000-1:256000, IHC:1:100-1:500, FC:1:100-1:300, IP:1µl-4µl |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Heat shock cognate 71 kDa protein (2-646aa) |
| Antigen Species | Human |
| Uniprot ID | P11142 |
| Target Name | HSPA8 |
| Clonality | Monoclonal |
| Clone ID | 2G8F6 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12927859/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse spleen tissue, Rat spleen tissue, Mouse heart tissue, Rat heart tissue
All lanes HSPA8 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 70~75 KDa
Observed band size: 70~75 KDa
Exposure time: 10s
Image 2
Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg
All lanes: HSPA8 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 70~75 KDa
Observed band size: 70~75 KDa
Exposure time: 10s
Image 3
Western Blot
Positive WB detected in: 20μg Hela whole cell lysate
HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 70~75 KDa
Observed band size: 70~75 KDa
Exposure time: 10s
Image 4
IHC image of CSB-MA010829A0m diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
Image 5
IHC image of CSB-MA010829A0m diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
Image 6
Immunoprecipitating HSPA8 in Hela whole cell lysate
Lane 1: Mouse control IgG2b instead of CSB-MA010829A0m in Hela whole cell lysate.
Lane 2: CSB-MA010829A0m (1.5µl) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
For western blotting, the blot was detected with CSB-MA010829A0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
Image 7
Overlay histogram showing MCF-7 cells stained with CSB-MA010829A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody at 1/200 for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b used under the same conditions. Acquisition of >10,000 events was performed.
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