Cusabio
SKU(재고 관리 코드):CSB-MA013563A0m
CD146 Monoclonal Antibody
CD146 Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA013563A0m |
| Short Name | CD146 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF, FC |
| Dilution | WB:1:500-1:2000, IF:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Cell surface glycoprotein MUC18 protein (50-646aa) |
| Antigen Species | Human |
| Uniprot ID | P43121 |
| Target Name | CD146 |
| Clonality | Monoclonal |
| Clone ID | 4D10C7 |
| Host Species | Mouse |
| Isotype | IgG2a |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12923069/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: CD146 antibody at 1:1250
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 72, 58 kDa
Observed band size: 120 kDa
Image 2
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate
All lanes: CD146 antibody at 1:500
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 72, 58 kDa
Observed band size: 120 kDa
Image 3
Immunofluorescence staining of A375 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 4
Immunofluorescence staining of Hela cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 5
Immunofluorescence staining of MCF-7 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 6
Immunofluorescence staining of THP-1 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 7
Overlay histogram showing MCF-7 cells stained with CSB-MA013563A0m (red line) at 1:400. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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