Cusabio
SKU(재고 관리 코드):CSB-MA0157131A0m
NES Monoclonal Antibody
NES Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA0157131A0m |
| Short Name | NES Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF, FC |
| Dilution | WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Nestin protein |
| Antigen Species | Human |
| Uniprot ID | P48681 |
| Target Name | NES |
| Clonality | Monoclonal |
| Clone ID | 2C2F7 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/1029016/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate
All lanes: NES antibody at 3µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 178 kDa
Observed band size: 260 kDa
Image 2
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-MA0157131A0m at dilution of 1:100
Image 3
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA0157131A0m at dilution of 1:100
Image 4
Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA0157131A0m at dilution of 1:100
Image 5
Immunofluorescent analysis of Hela cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 6
Immunofluorescent analysis of PC-3 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 7
Immunofluorescent analysis of U251 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 8
Overlay histogram showing U251 cells stained with CSB-MA0157131A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*10<sup>6</sup>cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
Image 9
Overlay histogram showing Hela cells stained with CSB-MA0157131A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*10<sup>6</sup>cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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